| Overview |
In Sanger sequencing, the quality of the peak shapes may not be good enough to identify the correct base in the beginning of the electropherogram. This is because the sieving matrix for electrophoresis (polymer) filled in the capillary may not be able to detect and separate short DNA properly. This report introduces an example of improving the data quality in the beginning of the electropherogram using a sequencing primer equipped with a tail on the DS3000 Compact CE Sequencer. |
