Overview |
The evolutionary history of microorganisms is recorded in ribosomal RNA (rRNA), and a method of phylogenetic classification has been adopted based on this record. In the 18th edition of the Japanese Pharmacopoeia, methods for microbial identification analysis using capillary sequencers are presented. These methods automatically analyze the gene sequence of part of the highly variable region of 16S rRNA for bacteria and the spacer region between 18S rRNA and 5.8S rRNA (ITS1) for fungi, and identify or estimate microorganisms by comparing them with a database. |