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Overview

SEM Pathology

Table of contents

1. Examination of common tissue samples

Staining properties of platinum blue

Staining properties of osmium tetroxide

2. Examination of renal biopsy samples

3. Advantages of LVSEMs and future tasks

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Staining properties of platinum blue　#000003

(2) Normal rat tongue tissue (high-magnification images)

Figure No.000003a

Staining: Platinum-blue staining

Microscope: LVSEM
Sample: Paraffin section

Accelerating voltage: 15 kV

Magnification: ×250
Scale bar: 100 μm

A: Arteriole　V: Vein　N: Nerve tissue　M: Muscle tissue　C: Connective tissue　Arrows: Mast cells

Image description
Segment of rat tongue tissue stained with platinum blue 4). Various tissue components can be clearly identified as distinct structures.

Figure No.000003b

Staining: Platinum-blue staining

Microscope: LVSEM
Sample: Paraffin section

Accelerating voltage: 15 kV

Magnification: ×1,000
Scale bar: 30 μm

Open arrow: Arterial endothelial cells. Small arrows: Internal elastic lamina.
＊: Smooth muscle layer of the tunica media. White arrow: Tunica adventitia.

Image description
A higher-magnification image of the arteriole boxed in Fig. 000003a. The three-layer (tunica intima, tunica media, tunica adventitia) structure of the wall of the arteriole is clearly delineated. In particular, endothelial cells are strongly stained and can be observed as the brightest areas.

Characteristic findings

In LVSEM images of animal tissue paraffin sections, cells and tissues can be identified based on their morphology and light-dark contrast (which is produced by differences in staining intensity). At higher magnifications, these images allow for a more detailed analysis of cell and tissue three-dimensional structures.

Observations

Fig. 000003a is a LVSEM image showing, at a slightly higher magnification (×250), part of the tissue section shown in Fig. 000002b. In this image, the cross-sectional morphologies of blood vessels (arterioles and veins), nerve tissue, muscle tissue, fibrous connective tissue, and various types of cells are clearly visible. In Fig. 000003b, a transverse section of an arteriole is shown at a higher magnification. Light-dark contrast is visible in the walls of blood vessels, clearly demonstrating the structural differences among the three layers of the wall.

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