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Overview

SEM Pathology

Table of contents

1. Examination of common tissue samples

Staining properties of platinum blue

Staining properties of osmium tetroxide

2. Examination of renal biopsy samples

3. Advantages of LVSEMs and future tasks

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Staining properties of platinum blue　#000004

(3) Mast cells in rat tongue tissue (light-microscope images, LVSEM images)

Figure No.000004a

Staining: Platinum-blue staining

Microscope: Light-microscope
Sample: Paraffin section

Magnification: ×400

Image description
Light-microscope image of mast cells in rat tongue tissue stained with platinum blue. The granules in the cytoplasm are stained dark blue.

Figure No.000004b

Staining: Platinum-blue staining

Microscope: LVSEM
Sample: Paraffin section

Accelerating voltage: 15 kV

Magnification: ×5,000

Image description
LVSEM image of mast cells in rat tongue tissue stained with platinum blue. The granules in the cells are exceptionally bright, and each granule can be observed clearly and in three dimensions.

Characteristic findings

The cytoplasm of mast cells contains many granules that are strongly stained by platinum blue. Consequently, these cells appear dark blue in light-microscope images, but are strikingly bright and clear under a LVSEM 3), 4). The three-dimensional shape of each granule in the cells becomes more evident when the cells are examined with a LVSEM at higher magnification. Even the size of the granules can be determined using the measurement mode of the LVSEM.

Observations

Mast cells appear dark blue in light-microscope Fig. 000004a, whereas they are extremely bright and prominent under a LVSEM. In higher-magnification LVSEM images, the mast cell cytoplasm filled with many granules appears three-dimensional.

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