Staining properties of platinum blue
Staining properties of osmium tetroxide
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Staining: Platinum-blue staining
Microscope: Light-microscopeSample: Paraffin section
Image descriptionLight-microscope image of mast cells in rat tongue tissue stained with platinum blue. The granules in the cytoplasm are stained dark blue.
Microscope: LVSEMSample: Paraffin section
Accelerating voltage: 15 kV
Image descriptionLVSEM image of mast cells in rat tongue tissue stained with platinum blue. The granules in the cells are exceptionally bright, and each granule can be observed clearly and in three dimensions.
The cytoplasm of mast cells contains many granules that are strongly stained by platinum blue. Consequently, these cells appear dark blue in light-microscope images, but are strikingly bright and clear under a LVSEM 3), 4). The three-dimensional shape of each granule in the cells becomes more evident when the cells are examined with a LVSEM at higher magnification. Even the size of the granules can be determined using the measurement mode of the LVSEM.
Mast cells appear dark blue in light-microscope Fig. 000004a, whereas they are extremely bright and prominent under a LVSEM. In higher-magnification LVSEM images, the mast cell cytoplasm filled with many granules appears three-dimensional.
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