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Overview

SEM Pathology

Table of contents

1. Examination of common tissue samples

Staining properties of platinum blue

Staining properties of osmium tetroxide

2. Examination of renal biopsy samples

3. Advantages of LVSEMs and future tasks

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(2) Comparison of platinum-blue and PAM staining images (low-magnification images)　#100003

LVSEM images of glomeruli (Platinum-blue staining, PAM staining)

Figure No.100003a

Staining: Platinum-blue staining

Microscope: LVSEM
Sample: Paraffin section (Membranous glomerulonephritis)

Accelerating voltage: 15 kV

Magnification: ×500

Image description
The entire glomerulus is brightly stained. Its morphology can be clearly observed, and each basic structure is discernible.

Figure No.100003b

Staining: PAM staining

Microscope: LVSEM
Sample: Paraffin section (Membranous glomerulonephritis)

Accelerating voltage: 15 kV

Magnification: ×600

Image description
PAM-positive components of the glomerulus, i.e., basement membranes, mesangial matrix, and collagen fibers, are especially bright and clear in this image.

Characteristic findings

Without staining, the basic structures of glomeruli cannot be clearly differentiated, as the entire tissue section appears dark. Platinum-blue and PAM can stain different types of basic structures of glomeruli, making them easy to recognize. In particular, PAM staining can identify disease-specific aberrations in the basement membrane. Thus, it is also routinely used for light microscopic examinations.

Observations

The images of glomeruli shown in Figs. 100003 a and b were acquired from different tissue sections. However, they were cut from the same paraffin block containing a biopsy specimen from a patient with membranous glomerulonephritis. A comparison of these two images clearly demonstrates the differences in the staining effects of platinum-blue and PAM.

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