Staining properties of platinum blue
Staining properties of osmium tetroxide
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Staining: Platinum-blue staining
Microscope: LVSEMSample: Paraffin section (Membranous glomerulonephritis)
Accelerating voltage: 15 kV
Image descriptionThe entire glomerulus is brightly stained. Its morphology can be clearly observed, and each basic structure is discernible.
Staining: PAM staining
Image descriptionPAM-positive components of the glomerulus, i.e., basement membranes, mesangial matrix, and collagen fibers, are especially bright and clear in this image.
Without staining, the basic structures of glomeruli cannot be clearly differentiated, as the entire tissue section appears dark. Platinum-blue and PAM can stain different types of basic structures of glomeruli, making them easy to recognize. In particular, PAM staining can identify disease-specific aberrations in the basement membrane. Thus, it is also routinely used for light microscopic examinations.
The images of glomeruli shown in Figs. 100003 a and b were acquired from different tissue sections. However, they were cut from the same paraffin block containing a biopsy specimen from a patient with membranous glomerulonephritis. A comparison of these two images clearly demonstrates the differences in the staining effects of platinum-blue and PAM.
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