Staining: PAM staining
Sample: Paraffin section (Membranous glomerulonephritis)
Accelerating voltage: 15 kV
P: Podocytes (Epithelial cells) E: Vascular endothelial cells M: Mesangial cells GBM: Glomerular basement membranes MM: Mesangial matrix L: Capillary lumen Bs: Bowman's space
LVSEM image 5) of a glomerulus from a patient with membranous glomerulonephritis stained with PAM. The glomerular basement membranes (GBM: arrows) and mesangial matrix (MM) are stained positive with PAM and are visualized as bright and distinct structures. The basement membranes show spike formation that is characteristic of membranous glomerulonephritis. Podocytes (epithelial cells, P) that line the outer surface of the glomerular capillaries, vascular endothelial cells (E), immune substances, and mesangial cells (M) are negative with PAM and thus appear dark and undefined in the image.