| HTB-DS-E016 | When using the DS3000 Compact CE Sequencer, the operator can choose the optimal polymer for the analysis from Polymer4 or Polymer7. Polymer7 is mainly used for sequencing analysis, but can also be used for fragment analysis. Here, we describe the performance of Polymer7 for fragment analysis using the GenePrintTM 24 System (Promega®). | 2024/01/22 |
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| HTB-DS-E015 | In Sanger sequencing, the quality of the peak shapes may not be good enough to identify the correct base in the beginning of the electropherogram. This is because the sieving matrix for electrophoresis (polymer) filled in the capillary may not be able to detect and separate short DNA properly. This report introduces an example of improving the data quality in the beginning of the electropherogram using a sequencing primer equipped with a tail on the DS3000 Compact CE Sequencer. | 2023/10/10 |
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| HTB-DS-E014 | Sequences rich in guanine (G) and cytosine (C) (GC-rich sequences) are often found in functionally important positions on the genome such as transcription control regions. GC-rich sequences inhibit polymerase extension reactions, resulting in failure to obtain expected products in the polymerase chain reaction (PCR) and the cycle sequencing reaction. | 2023/10/10 |
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| HTB-DS-E013 | The MLPA® method (Reagent Manufacturer: MRC Holland, Reagent Distributor inJapan: FALCO biosystems Ltd.) is widely used to analyze copy number variation (deletions/duplications: CNV), known SNP, and methylation status. Sequences that specifically hybridize to the target gene are designed within the MLPA® probe, and mutations are analyzed by capillary electrophoresis through hybridization, ligation, and PCR. An example of MLPA® analysis using the DS3000 Compact CE Sequencer (DS3000) is presented here. | 2023/05/22 |
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| HTB-DS-E012 | A new function has been added to the DS3000 Compact CE Sequencer (DS3000). With the new DS3000, you can now customize the fluorescent dyes and size standards used in your analysis. You can also use more reagent kits. As an example of the enhanced utility of the DS3000 with the added function, SNaPshotTM (Thermo Fisher Scientific) was performed using a combination of fluorophores that was not supported by the previous DS3000. | 2023/05/22 |
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| HTB-DS-E010 | Due to the ease of the analysis, genomic variations detected by next-generation sequencing (NGS variants) are sometimes confirmed by Sanger sequencing (1). Here, we introduce a practical example of confirming NGS variants using the DS3000 Compact CE Sequencer (DS3000). | 2022/08/24 |
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| HTB-DS-E009 | Rare variants are mutations that are found in a small subpopulation of specimens. They have been recognized for their usefulness in cancer research, because they can cause tumorigenesis and promote tumor progression. The signals from rare variants are buried behind the signals from wild-type DNA, which comprises the vast majority of DNA in samples. Therefore, the detection of rare variants requires very high sensitivity and accuracy. | 2022/06/02 |
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| HTB-DS-E008 | Of the four bases that make up genomic DNA in higher eukaryotes, the fifth carbon of cytosine can be methylated by a methyltransferase. Cytosine methylation plays an important role in various events that occur on genomic DNA, including the regulation of gene expression, and it is known as a representative epigenetic marker. | 2022/06/02 |
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| HTB-DS-E007 | Cell line contamination, misidentification, and genetic drift result in invalid research. It has been estimated that 18% to 36% of cell lines are contaminated or misidentified (1). To address this issue, a number of scientific journals request researchers to perform cell line authentication (CLA) prior to submitting a manuscript that involves the use of cell lines (2). | 2022/06/02 |
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| HTB-DS-E006 | With the DS3000 Compact CE Sequencer (hereinafter, the “DS3000”), DNA molecules are injected into a capillary array as a result of the difference in electrical potential between the anode and the negatively charged DNA molecules. The magnitude and duration of the applied voltage (hereinafter , the “injection voltage” and “injection time”, respectively) have a significant effect on the number of molecules injected and the peak resolution,thereby affecting the accuracy of electrophoresis measurements. | 2022/03/15 |
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| HTB-DS-E005 | When using the DS3000 Compact CE Sequencer, the operator can choose the optimal polymer for the analysis from Polymer4 or Polymer7. Polymer7 is mainly used for sequencing analysis, but can also be used for fragment analysis. Here, we describe the performance of Polymer7 for fragment analysis using the GenePrintTM 24 System (Promega®). | 2021/10/28 |
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| HTB-DS-E004 | Food allergies occur in approximately 1-3% of adults and 4-6% of children [1] and occasionally can cause life-threatening symptoms. Thus, detection of food allergens and labeling of them on food products are critical issue for food safety. Although protein allergen can be detected directly by a conventional method such as ELISA, nucleic acid-based assay, which enables detect DNA sequences coding allergens, is sometimes employed because of its higher sensitivity, less cost and better ease-of-use. | 2022/04/22 |
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| HTB-DS-E003 | Microsatellite instability (MSI), an accumulation of somatic mutations in microsatellites or short tandem repeat sequences, is a consequence of DNA mismatch repair (MMR) system deficiency, and thus considered as a biomarker for multiple types of cancers. In MSI analysis, DNA fragments were distributed in single-base resolution and compared with the reference data on a capillary electrophoresis (CE). | 2022/04/22 |
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